//M1//QN1//MCQ//DL0

In a DNA strand the nucleotides are linked together by :

(A) glycosidic bonds (B) phosphodiester bonds (C) peptide bonds (D) hydrogen bonds

//X

(B) phosphodiester bonds

//M1//QN2//MCQ//DL0

A nucleoside differs from a nucleotide. It lacks the :

(A) base (B) sugar (C) phosphate group (D) hydroxyl group

//X

(C) phosphate group

//M1//QN3//MCQ//DL0

Both deoxyribose and ribose belong to a class of sugars called:

(A) trioses (B) hexoses (C) pentoses (D) polysaccharides

//X

(C) pentoses

//M1//QN4//MCQ//DL0

The fact that a purine base always pairs through hydrogen bonds with a pyrimidine base in the DNA double helix leads to :

(A) the antiparallel nature (B) the semiconservative nature (C) uniform width throughout DNA (D) uniform length in all DNA

//X

(C) uniform width throughout DNA

//M1//QN5//MCQ//DL0

Which of the following is true with respect to AUG?

(A) It codes for methionine only (B) It is an initiation codon (C) It codes for methionine in both prokaryotes and eukaryotes (D) All of the above

//X

(D) All of the above

//M1//QN6//MCQ//DL0

The first genetic material could be :

(A) protein (B) carbohydrates (C) DNA (D) RNA

//X

(D) RNA

//M1//QN7//MCQ//DL0

Discontinuous synthesis of DNA occurs in one strand, because:

(A) DNA molecule being synthesized is very long. (B) DNA dependent DNA polymerase catalyses polymerisation only in one direction
(5’ → 3’). (C) It is a more efficient process. (D) DNA ligase joins the short stretches of DNA.

//X

(B) DNA dependent DNA polymerase catalyses polymerisation only in one direction
(5’ → 3’).

//M1//QN8//MCQ//DL0

The net electric charge on DNA and histones is:

(A) both positive (B) both negative (C) negative and positive, respectively (D) zero

//X

(C) negative and positive, respectively

//M1//QN9//MCQ//DL0

The promoter site and the terminator site for transcription are located at :

(A) 3' (downstream) end and 5' (upstream) end, respectively of the transcription unit (B) 5' (upstream) end and 3' (downstream) end, respectively of the transcription unit (C) the 5' (upstream) end (D) the 3' (downstream) end

//X

(B) 5' (upstream) end and 3' (downstream) end, respectively of the transcription unit

//M1//QN10//MCQ//DL0

The human chromosome with the highest and least number of genes in them are respectively:

(A) Chromosome 21 and Y (B) Chromosome 1 and X (C) Chromosome 1 and Y (D) Chromosome X and Y

//X

(C) Chromosome 1 and Y

//M1//QN11//MCQ//DL0

In some viruses, DNA is synthesized by using RNA as template. Such a DNA is called:

(A) A-DNA (B) B-DNA (C) cDNA (D) rDNA

//X

(C) cDNA

//M1//QN12//MCQ//DL0

With regards to mature mRNA in eukaryotes:

(A) exons and introns do not appear in the mature RNA (B) exons appear but introns do not appear in the mature RNA (C) introns appear but exons do not appear in the mature RNA (D) both exons and introns appear in the mature RNA

//X

(B) exons appear but introns do not appear in the mature RNA

//M1//QN13//MCQ//DL0

Who amongst the following scientists had no contribution in the development of the double helix model for the structure of DNA?

(A) Rosalind Franklin (B) Maurice Wilkins (C) Erwin Chargaff (D) Meselson and Stahl

//X

(D) Meselson and Stahl

//M1//QN14//MCQ//DL0

DNA is a polymer of nucleotides which are linked to each other by 3’-5’ phosphodiester bond. To prevent polymerisation of nucleotides, which of the following modifications would you choose?

(A) Replace purine with pyrimidines (B) Remove\ Replace 3' OH group in deoxy ribose (C) Remove\ Replace 2' OH group with some other group in deoxy ribose (D) Both ‘B’ and ‘C’

//X

(B) Remove\ Replace 3' OH group in deoxy ribose

//M1//QN15//MCQ//DL0

Which of the following steps in transcription is catalysed by RNA polymerase?

(A) Initiation (B) Elongation (C) Termination (D) All of the above

//X

(B) Elongation

//M1//QN16//MCQ//DL0

Control of gene expression in prokaryotes take place at the level of:

(A) DNA-replication (B) Transcription (C) Translation (D) None of the above

//X

(B) Transcription

//M1//QN17//MCQ//DL0

Which of the following statements is correct about the role of regulatory proteins in transcription in prokaryotes?

(A) They only increase expression. (B) They only decrease expression. (C) They interact with RNA polymerase but do not affect the expression. (D) They can act both as activators and as repressors.

//X

(D) They can act both as activators and as repressors.

//M1//QN18//MCQ//DL0

Which was the last human chromosome to be completely sequenced ?

(A) Chromosome 1 (B) Chromosome 11 (C) Chromosome 21 (D) Chromosome X

//X

(A) Chromosome 1

//M1//QN19//MCQ//DL0

The amino acid attaches to the tRNA at its:

(A) 5' - end (B) 3' - end (C) Anti codon site (D) DHU loop

//X

(B) 3' - end

//M1//QN20//MCQ//DL0

Which of the following is the function of RNA?

(A) It is a carrier of genetic information from DNA to ribosomes synthesising polypeptides. (B) It carries amino acids to ribosomes. (C) It is a constituent component of ribosomes. (D) All of the above.

//X

(D) All of the above.

//M1//QN21//MCQ//DL0

While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were: Adenine = 29%, Guanine = 17%, Cytosine = 32%, Thymine = 17%. Considering the Chargaff’s rule it can be concluded that :

(A) It is a double stranded circular DNA. (B) It is single stranded DNA. (C) It is a double stranded linear DNA. (D) No conclusion can be drawn.

//X

(B) It is single stranded DNA.

//M1//QN22//MCQ//DL0

If Meselson and Stahl's experiment is continued for four generations in bacteria, the ratio of N 15/N 15 : N 15/N 14 : N 14/N 14 containing DNA in the fourth generation would be :

(A) 1 : 1 : 0 (B) 1 : 4 : 0 (C) 0 : 1 : 3 (D) 0 : 1 : 7

//X

(B) 1 : 4 : 0

//M1//QN23//MCQ//DL0

If the sequence of nitrogen bases of the coding strand of DNA in a transcription unit is : 5' - A T G A A T G - 3', the sequence of bases in its RNA transcript would be;

(A) 5' - A U G A A U G - 3' (B) 5' - U A C U U A C - 3' (C) 5' - C A U U C A U - 3' (D) 5' - G U A A G U A - 3'

//X

(A) 5' - A U G A A U G - 3'

//M1//QN24//MCQ//DL0

The RNA polymerase holoenzyme transcribes:

(A) the promoter, structural gene and the terminator region (B) the promoter and the terminator region (C) the structural gene and the terminator region (D) the structural gene only

//X

(D) the structural gene only

//M1//QN25//MCQ//DL0

If the base sequence of a codon in mRNA is 5'-AUG-3', the sequence of tRNA pairing with it must be:

(A) 5' - UAC - 3' (B) 5' - CAU - 3' (C) 5' - AUG - 3' (D) 5' - GUA - 3'

//X

(B) 5' - CAU - 3'

//M1//QN26//MCQ//DL0

To initiate translation, the mRNA first binds to :

(A) The smaller ribosomal sub-unit (B) The larger ribosomal sub-unit (C) The whole ribosome (D) No such specificity exists.

//X

(A) The smaller ribosomal sub-unit

//M1//QN27//MCQ//DL0

In E.coli, the lac operon gets switched on when:

(A) lactose is present and it binds to the repressor. (B) repressor binds to operator. (C) RNA polymerase binds to the operator. (D) lactose is present and it binds to RNA polymerase.

//X

(A) lactose is present and it binds to the repressor.

//M1//QN28//MCQ//DL0

Chargaff's rules is regarding ________.

(A) Composition of bases in RNA (B) Number of chromosomes (C) Composition of bases in DNA (D) complexity of genetic material

//X

(C) Composition of bases in DNA

//M1//QN29//MCQ//DL0

If the total number of (T) is 77 then the total number of (C) will be : ________.

(A) 77 (B) 100 (C) 154 (D) Can't say

//X

(D) Can't say

//M1//QN30//MCQ//DL0

Nucleotides are building blocks of nucleic acids. Each nucleotide is a composite molecule formed by

(A) (Base − sugar − phosphate) N (B) Base-sugar −OH (C) Base-sugar-phosphate (D) Sugar-phosphate

//X

(C) Base-sugar-phosphate

//M1//QN31//MCQ//DL0

Who among the following proposed rules regarding the composition of bases in DNA?

(A) Watson Crick (B) Erwin Chargaff (C) Dr. Hargovind Khorana (D) Griffith

//X

(B) Erwin Chargaff

//M1//QN32//MCQ//DL0

The first rule of Chargaff :

(A) A + G = T + C (B) A+ T = G + C (C) A + C = T + C (D) A= G

//X

(A) A + G = T + C

//M1//QN33//MCQ//DL0//EQ

It varies with the organism.

(A) A + G = T + C (B) A= G (C) A = T and G = C (D)

//X

(D)

//M1//QN34//MCQ//DL0

If the total amount of Adenine (A) and guanine (G) is 100 % then the total amount of thymine (T) and cytosine (C) will be ________.

(A) 50% (B) 75% (C) 25% (D) 100%

//X

(D) 100%

//M1//QN35//MCQ//DL0

In DNA if 10% guanine is present, how much is thymine present?

(A) 10% (B) 40% (C) 80% (D) 20%

//X

(B) 40%

//M1//QN36//MCQ//DL0

The unidirectional flow of genetic information from DNA → m – RNA → Protein replication is known as ________.

(A) Transcription (B) Replication (C) Central dogma (D) Translation

//X

(C) Central dogma

//M1//QN37//MCQ//DL0

Reverse transcription means ________.

(A) Formation of m- RNA from DNA (B) Formation of DNA from RNA (C) Formation of DNA from DNA (D) Formation of t-RNA from m-RNA

//X

(B) Formation of DNA from RNA

//M1//QN38//MCQ//DL0

DNA polymerase links nucleotide by forming which type of bond?

(A) Phosphodiester bond (B) Hydrogen bond (C) Hydrogen bond (D) Ester bond

//X

(A) Phosphodiester bond

//M1//QN39//MCQ//DL0

Which of the following is a nucleoside?

(A) Adenosine, Adenylic acid, Cytosine (B) Adenosine, Guanosine, Cytidine (C) Cytidylic acid, Adenosine, Adenylic acid (D) Guanylic acid, Cytosine, Adenosine

//X

(B) Adenosine, Guanosine, Cytidine

//M1//QN40//MCQ//DL0

Thirty percent of the bases in a sample of DNA extracted from eukaryotic cells is adenine. What percentage of cytosine is present in this DNA?

(A) 10% (B) 20% (C) 30% (D) 40%

//X

(B) 20%

//M1//QN41//MCQ//DL0

If a length of DNA has 45,000 base pairs,

how many complete turns will the DNA molecule take?

(A) 4,500 (B) 45,000 (C) 45 (D) 450

//X

(A) 4,500

//M1//QN42//MCQ//DL0

If Adenine makes 30% of the DNA molecule, what will be the percentage of Thymine, Guanine and Cytosine in it?

(A) T : 20; G : 30; C : 20 (B) T : 20; G : 20; C : 30 (C) T : 30; G : 20; C : 20 (D) T : 20; G : 25; C : 25

//X

(C) T : 30; G : 20; C : 20

//M1//QN43//MCQ//DL0

One turn of the helix in a B form DNA is approximately

(A) 2 nm (B) 20 nm (C) 0.34 nm (D) 3.4 nm

//X

(D) 3.4 nm

//M1//QN44//MCQ//DL0

Who among the following did the experiment of bacterial transformation?

(A) Avery, McCarty, Macleod (B) Griffith (C) Hershey, Chase (D) Watson, Crick

//X

(B) Griffith

//M1//QN45//MCQ//DL0

Griffith's experiment is famous for ________.

(A) viral transformation (B) pneumococcus transduction (C) bacterial transformation (D) bacterial recombination

//X

(C) bacterial transformation

//M1//QN46//MCQ//DL0

The organism used in Griffith's experiment was ________.

(A) Spirulina (B) Bacillus (C) Pneumococcus (D) Salmonella

//X

(C) Pneumococcus

//M1//QN47//MCQ//DL0

Griffith had performed series of experiments on

(A) Pneumococcus (B) Bacillus (C) Vibrio bacteria (D) Spirilium bacteria

//X

(A) Pneumococcus

//M1//QN48//MCQ//DL0

Which of the following is incorrect for Hershey - Chase experiment?

(A) Infection (B) Blending (C) Centrifugation (D) PCR

//X

(D) PCR

//M1//QN49//MCQ//DL0

Which of the following is not correct for molecule that act as a genetic material?

(A) It should be able to generate its replica (B) It should be structurally stable (C) It should be able to express itself in the form of Mendelian characters (D) It should be Heat resistant

//X

(D) It should be Heat resistant

//M1//QN50//MCQ//DL0

The unequivocal proof of DNA as the genetic material came from the studies on a

(A) bacterium (B) fungus (C) viroid (D) bacterial virus

//X

(D) bacterial virus

//M1//QN51//MCQ//DL0

Taylor conducted the experiments to prove semiconservative mode of chromosome replication on

(A) Vinca rosea (B) Vicia faba (C) Drosophila melanogaster (D) E. coli.

//X

(B) Vicia faba

//M1//QN52//MCQ//DL0

The term 'Nuclein' for the genetic material was used by

(A) Mendel (B) Franklin (C) Meischer (D) Chargaff

//X

(C) Meischer

//M1//QN53//MCQ//DL0

DNA strand is directly involved in the synthesis of all of the following except

(A) tRNA molecule (B) mRNA molecule (C) Another DNA strand (D) Protein synthesis

//X

(D) Protein synthesis

//M1//QN54//MCQ//DL0

The association of histone H 1 with a nucleosome indicates that

(A) DNA replication is occurring (B) the DNA is condensed into a chromatin fibre (C) the DNA double helix is exposed (D) transcription is occurring.

//X

(B) the DNA is condensed into a chromatin fibre

//M1//QN55//MCQ//DL0

The expression of the genetic material which occurs generally through ________.

(A) production of carbohydrates (B) production of protein (C) accumulation of genetic material (D) production of lipids

//X

(B) production of protein

//M1//QN56//MCQ//DL0

Which scientist experimentally proved that DNA is the sole genetic material in bacteriophage?

(A) Beadle and Tautum (B) Meselson and Stahl (C) Hershey and Chase (D) Jacob and Monod

//X

(C) Hershey and Chase

//M1//QN57//MCQ//DL0

DNA is the genetic material because

(A) DNA is the chemically and structurally stable material. (B) It is having characteristic of self replication. (C) It is Expressed in the form of Mendelian characteristics. (D) All are the true.

//X

(D) All are the true.

//M1//QN58//MCQ//DL0

Which enzyme is responsible for reverse transcription ?

(A) Reverse polymerase (B) Reverse transcriptase (C) Reverse ligase (D) Reverse DNase

//X

(B) Reverse transcriptase

//M1//QN59//MCQ//DL0

An experiment was carried out in which a hybrid DNA was allowed to replicate for one generation in medium containing 15 NH 4 Cl and for second generation in medium containing 14 NH 4 Cl Which of the given conclusions can be drawn based on above stated experiment? A. Percentage of hybrid DNA obtained in both the generations are equal B. 50% of heavy DNA are obtained in first generation. C. 25% of light DNA are obtained in second generation D. 25 % of heavy DNA are obtained in second generation

(A) A & B (B) B & C (C) C & D (D) A & D

//X

(B) B & C

//M1//QN60//MCQ//DL0

The total number of nitrogenous bases in human genome is estimated to be about

(A) 3.5 million (B) 35 thousand (C) 35 million (D) 3.1 billion

//X

(C) 35 million

//M1//QN61//MCQ//DL0

Reverse transcription is observed in _______.

(A) TMV (B) Tumor viruses (C) HIV (D) All of the above

//X

(D) All of the above

//M1//QN62//MCQ//DL0

Identify the incorrect statement about RNA.

(A) RNA was the first genetic material to evolve in the living systems. (B) Apart from being a genetic material, it also acts as catalyst. (C) DNA evolved from RNA with chemical modifications. (D) RNA being a catalyst is non-reactive and stable.

//X

(D) RNA being a catalyst is non-reactive and stable.

//M1//QN63//MCQ//DL0

Which of the following enzymes is non-proteinaceous ?

(A) Deoxyribonuclease (B) Ligase (C) Ribozyme (D) Lysozyme

//X

(C) Ribozyme

//M1//QN64//MCQ//DL0

Which of the following is found more widely in a cell?

(A) RNA (B) DNA (C) Sphaerosomes (D) Chloroplasts

//X

(A) RNA

//M1//QN65//MCQ//DL0

RNA contains repeating units of

(A) deoxyribonucleotides (B) ribonucleotides (C) deoxyribonucleosides (D) ribonucleosides

//X

(B) ribonucleotides

//M1//QN66//MCQ//DL0

RNA is present as a genetic material in ________.

(A) TMV virus (B) Cyanobacteria (C) Cladophora (D) Sieve tube cells

//X

(A) TMV virus

//M1//QN67//MCQ//DL0

Which of the following is not a protein?

(A) Abzyme (B) Ribozyme (C) DNA ligase (D) DNA gyrase

//X

(B) Ribozyme

//M1//QN68//MCQ//DL0

Consider the following statements, (i) RNA was the first genetic material (ii) RNA is more reactive (iii) RNA is unstable as compared to DNA Which statement is correct?

(A) (i) (B) (ii) (C) (i), (ii) and (iii) (D) None of the above

//X

(C) (i), (ii) and (iii)

//M1//QN69//MCQ//DL0

Choose the correct statement about the direction of coding strand.

(A) 3' → 5' (B) 2' → 5' (C) 5' → 3' (D) 5' → 5' and 3' → 3'

//X

(C) 5' → 3'

//M1//QN70//MCQ//DL0

It stimulates DNA replication :

(A) DNA polymerase (B) DNA Ligase (C) Transcriptase (D) Both (A) and (B)

//X

(D) Both (A) and (B)

//M1//QN71//MCQ//DL0

The direction and the type of DNA replication is ________.

(A) Bidirectional and conservative (B) Unidirectional and conservative (C) Bidirectional and semiconservative (D) Unidirectional and Semiconservative

//X

(C) Bidirectional and semiconservative

//M1//QN72//MCQ//DL0

Choose the correct option for formation of okazaki fragments.

(A) Replication (B) Transcription (C) Translation (D) Transduction

//X

(A) Replication

//M1//QN73//MCQ//DL0

What is a primer?

(A) long strand of DNA (B) short strand of DNA (C) long chain of RNA (D) short RNA chain

//X

(D) short RNA chain

//M1//QN74//MCQ//DL0

When DNA replication will start?

(A) When leading strand produce okazaki fragments. (B) When hydrogen bonds between two polynucleotides chains are sequentially broken with the help of proper enzymes. (C) When phosphodiester bonds between sequentially two nucleotides are broken. (D) When bonds between pentose sugar and Nitrogen base are broken.

//X

(B) When hydrogen bonds between two polynucleotides chains are sequentially broken with the help of proper enzymes.

//M1//QN75//MCQ//DL0

Enzyme that breaks the bond between DNA helix is known as ________.

(A) Helicase (B) Gyrase (C) Ligase (D) DNA polymerase

//X

(A) Helicase

//M1//QN76//MCQ//DL0

Ligase joins nucleotides with ________.

(A) glycosidic bond (B) ester bond (C) peptide bond (D) phosphodiester bond

//X

(D) phosphodiester bond

//M1//QN77//MCQ//DL0

Okazaki fragment were formed

(A) On the continuos strand at a time of DNA replication. (B) On the discontinuous strand at a time of DNA replication. (C) At a time of transcription. (D) From the introns at a time of transcription.

//X

(B) On the discontinuous strand at a time of DNA replication.

//M1//QN78//MCQ//DL0

Antiparallel strands of a DNA molecule means that

(A) one strand turns clockwise (B) one strand turns anticlockwise (C) the phosphate groups of twoDNA strands, at their ends, share the same position (D) the phosphate groups at the start of two DNA strands are in opposite position (pole).

//X

(D) the phosphate groups at the start of two DNA strands are in opposite position (pole).

//M1//QN79//MCQ//DL0

At the end of DNA replication, DNA molecule is having

(A) One strand of parental DNA and second is newly formed. (B) Both strands are newly synthesized. (C) Two strands are of parental DNA. (D) Both strands are different from parental DNA.

//X

(A) One strand of parental DNA and second is newly formed.

//M1//QN80//MCQ//DL0

In the DNA molecule,

(A) the proportion of adenine in relation to thymine varies with the organism (B) there are two strands which run antiparallel one in 5' → 3' direction and other in 3' → 5' (C) the total amount of purine nucleotides and pyrimidine nucleotides is not always equal (D) there are two strands which run parallel in the 5' → 3' direction.

//X

(B) there are two strands which run antiparallel one in 5' → 3' direction and other in 3' → 5'

//M1//QN81//MCQ//DL0

During replication of a bacterial chromosome DNA synthesis starts from a replication origin site and

(A) Moves in one direction of the site (B) Moves in bi-directional way (C) RNA primers are involved (D) Move in unidirectional way

//X

(B) Moves in bi-directional way

//M1//QN82//MCQ//DL0

Select the correct option.

| Direction of RNA Synthesis | Direction of reading of the template DNA
(a) | 5' – 3' | 3' – 5'
(b) | 3' – 5' | 5' – 3'
(c) | 5' – 3' | 5' – 3'
(d) | 3' – 5' | 3' – 5'

(A) A and R both are correct, and R is correct explanation of A. (B) A and R both are correct, but R is not correct explanation of A. (C) A is correct, but R is wrong (D) A is wrong, but R is correct

//X

(A) A and R both are correct, and R is correct explanation of A.

//M1//QN83//MCQ//DL0

Which enzyme is responsible for transcription?

(A) DNA polymerase (B) RNA polymerase (C) Transcriptase (D) All of the above

//X

(C) Transcriptase

//M1//QN84//MCQ//DL0

Which RNA is synthesized by transcription?

(A) m-RNA (B) r-RNA (C) t-RNA (D) All of the above

//X

(A) m-RNA

//M1//QN85//MCQ//DL0

Enzyme responsible for the formation of phosphodiester bond in the process of transcription is ______.

(A) DNA polymerase-III (B) DNA Ligase (C) DNA helicase (D) None of the above

//X

(D) None of the above

//M1//QN86//MCQ//DL0

At the end of transcription m-RNA is translocated to ________.

(A) Nucleus (B) Cytoplasm (C) Mitochondria (D) One cell to another cell

//X

(B) Cytoplasm

//M1//QN87//MCQ//DL0

m-RNA is translocated to cytoplasm where it associates with _________ .

(A) Chloroplast (B) Mitochondria (C) Ribosomes (D) Golgibody

//X

(C) Ribosomes

//M1//QN88//MCQ//DL0

Nucleoid is present in

(A) Plant cell (B) Prokaryotic cell (C) Animal cell (D) Eukaryotic cell

//X

(B) Prokaryotic cell

//M1//QN89//MCQ//DL0

Which one of the following makes use of RNA as a template to synthesize DNA?

(A) Reverse transcriptase (B) DNA dependant RNA polymerase (C) DNA polymerase (D) RNA polymerase

//X

(A) Reverse transcriptase

//M1//QN90//MCQ//DL0

Choose the correct statement.

(A) Transcription and translation occur in the same compartment in prokaryotes (B) Monocistonic RNA - more than one structural genes under single promoter (C) Introns and exons both code for protein synthesis (D) In prokaryotes, splicing and tailing occurs before translation.

//X

(A) Transcription and translation occur in the same compartment in prokaryotes

//M1//QN91//MCQ//DL0

Which of the following RNAs picks up specific amino acid (from amino acid pool) in the cytoplasm to ribosome during protein synthesis?

(A) tRNA (B) mRNA (C) rRNA (D) All of the above

//X

(A) tRNA

//M1//QN92//MCQ//DL0

Match the following RNA polymerase with their transcribed products Select the correct option from the following

Column : I | Column : II
a | RNA polymerase I | i | tRNA
b | RNA polymerase II | ii | rRNA
c | RNA polymerase III | iii | hnRNA

(A) a – i, b – iii, c – ii (B) a – i, b – ii, c – iii (C) a – ii, b – iii, c – i (D) a – i, b – iii, c – ii

//X

(C) a – ii, b – iii, c – i

//M1//QN93//MCQ//DL0

In capping ________ is added to the 5' end of hnRNA.

(A) Methyl guanosine triphosphate (B) Methyl adenosine triphosphate (C) Methyl cytidine triphosphate (D) Methyl uridine triphosphate

//X

(A) Methyl guanosine triphosphate

//M1//QN94//MCQ//DL0

Which type of RNA is most abundant in cell?

(A) mRNA (B) tRNA (C) rRNA (D) catalytic RNA

//X

(C) rRNA

//M1//QN95//MCQ//DL0

The phase of gene expression is ________.

(A) Transcription (B) Translation (C) Transduction (D) Both (A) and (B)

//X

(D) Both (A) and (B)

//M1//QN96//MCQ//DL0

Removal of introns and joining the exons in a defined order in a transcription unit is called

(A) tailing (B) transformation (C) capping (D) splicing

//X

(D) splicing

//M1//QN97//MCQ//DL0

DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the

(A) template strand (B) coding strand (C) alpha strand (D) antistrand

//X

(A) template strand

//M1//QN98//MCQ//DL0

The secret of the blue print of genetic information lies in the arrangement of ________.

(A) definite linear sequence of nitrogen bases of a RNA (B) definite linear sequence of nitrogen bases of a DNA (C) on the quality of nitrogen bases (D) number of purine and pyrimidine bases

//X

(B) definite linear sequence of nitrogen bases of a DNA

//M1//QN99//MCQ//DL0

Whose experiments cracked the DNA and discovered unequivocally that a genetic code is a "triplet?"

(A) Griffith (B) Watson, crick (C) Nirenberg, Matthaei and Khorana (D) Tschermak

//X

(C) Nirenberg, Matthaei and Khorana

//M1//QN100//MCQ//DL0

What is genetic code?

(A) The information present in the m-RNA is transcribed and carried by the DNA to the cytoplasm for protein synthesis. (B) The sequence of nitrogen base in t-RNA for synthesis of protein. (C) The sequence of nitrogen base in r-RNA molecule which contains information for Ribosomes. (D) The sequence of nitrogen bases in m-RNA molecule which contains the information for the synthesis of protein molecule.

//X

(D) The sequence of nitrogen bases in m-RNA molecule which contains the information for the synthesis of protein molecule.

//M1//QN101//MCQ//DL0

Any one codon specifies the position of one kind of amino-acid only ________.

(A) specific (B) universal (C) degenerate (D) non-sense

//X

(A) specific

//M1//QN102//MCQ//DL0

How many codons, codes for amino acids?

(A) 4 (B) 61 (C) 64 (D) 3

//X

(B) 61

//M1//QN103//MCQ//DL0

Which codons cause chain termination of protein synthesis ?

(A) AUG, UAG, UGA (B) UAA, UGA, UAG (C) UAA, GAU, GUC (D) AUG, UAA, UCA

//X

(B) UAA, UGA, UAG

//M1//QN104//MCQ//DL0

How many non-sense codons are there?

(A) 20 (B) 61 (C) 3 (D) 1

//X

(C) 3

//M1//QN105//MCQ//DL0

What is degenerate codons?

(A) One code which determine the same amino acid in all organisms. (B) Any one codon specifies the position of one kind of amino acid only. (C) A single amino acid may be specified by many codons. (D) Codons do not code for any amino-acid.

//X

(C) A single amino acid may be specified by many codons.

//M1//QN106//MCQ//DL0

Which of the following codon codes for methionine?

(A) AUG (B) UAG (C) CCC (D) AGU

//X

(A) AUG

//M1//QN107//MCQ//DL0

Which one of the following codons code for serine?

(A) UCU, UCC, UCA, UCG (B) UUU, UUC, UUA, UUG (C) CUU, CUC, CUA, CUG (D) CCU, CCC, CCA, CCG

//X

(A) UCU, UCC, UCA, UCG

//M1//QN108//MCQ//DL0

Which one of the following codons code for lysine ?

(A) AAA, AAG (B) AGU, AGC (C) GGU, GGC (D) UUA, UUG

//X

(A) AAA, AAG

//M1//QN109//MCQ//DL0

How many codons code for serine amino acid?

(A) 4 (B) 2 (C) 8 (D) 6

//X

(D) 6

//M1//QN110//MCQ//DL0

The function of t-RNA is

(A) To provide information for synthesis of protein. (B) To transport Amino acid molecules of various kinds located in cytoplasm. (C) To transport Amino acid from cytoplasm to nucleus. (D) To act as a template strand in the process of m-RNA synthesis.

//X

(B) To transport Amino acid molecules of various kinds located in cytoplasm.

//M1//QN111//MCQ//DL0

How many effective nucleotides are present in t-RNA?

(A) 25 to 30 (B) 80 to 90 (C) 75 to 95 (D) More than 95

//X

(C) 75 to 95

//M1//QN112//MCQ//DL0

How many types of t-RNA are present in cytoplasm?

(A) 61 (B) 20 (C) 64 (D) 16

//X

(A) 61

//M1//QN113//MCQ//DL0

A specific nucleotide sequence attached to 3' end of t-RNA is ________.

(A) AAC (B) CCA (C) AUG (D) UUA

//X

(B) CCA

//M1//QN114//MCQ//DL0

The site for amino acid attachment on t-RNA is ________.

(A) Anticodon region (B) On TψC 100P (C) On D LOOP (D) At 3–- OH end

//X

(D) At 3–- OH end

//M1//QN115//MCQ//DL0

During protein synthesis in an organism, at one point the process comes to a halt. Select the group of the three codons from the following, from which anyone of the three could bring about this halt.

(A) UUU, UCC, UAU (B) UUC, UUA, UAC (C) UAG, UGA, UAA (D) UUG, UCA, UCG

//X

(C) UAG, UGA, UAA

//M1//QN116//MCQ//DL0

Select the correct option regarding genetic code of Glycine.

(A) GUU, GUC, GUA (B) GAU, GAC, GAA (C) GGU, GGA, GGC (D) GGU, GGA,GCU

//X

(C) GGU, GGA, GGC

//M1//QN117//MCQ//DL0

Which form of RNA has a structure resembling clover leaf?

(A) mRNA (B) tRNA (C) rRNA (D) hn RNA

//X

(B) tRNA

//M1//QN118//MCQ//DL0

Which one of the following group of codons it is not called as degenerate codons?

(A) UAA, UAG and UGA (B) GUA, GUG and GCA (C) UUC, UUG and CCU, (D) UUA, UUG and CUU

//X

(A) UAA, UAG and UGA

//M1//QN119//MCQ//DL0

Out of 64 codons, 61 codons code for 20 types of amino acid. It is called

(A) Wobbling of codon (B) Overlapping of gene (C) Universility of codons (D) Degeneracy of genetic code

//X

(D) Degeneracy of genetic code

//M1//QN120//MCQ//DL0

Which of the following is the simplest amino acid?

(A) Tyrosine (B) Proline (C) Glycine (D) Glutamic acid

//X

(C) Glycine

//M1//QN121//MCQ//DL0

Anticodon is

(A) Paired triplet of bases on messenger RNA (B) Unpaired triplet of bases on rRNA (C) Paired triplet of bases on rRNA (D) An unpaired triplet of bases in an exposed position of tRNA

//X

(D) An unpaired triplet of bases in an exposed position of tRNA

//M1//QN122//MCQ//DL0

Identify the basic amino acid from the following.

(A) Valine (B) Tyrosine (C) Glutamic Acid (D) Lysine

//X

(D) Lysine

//M1//QN123//MCQ//DL0

There are 64 types of codons in genetic code dictionary because

(A) There are 64 types of tRNA’s found in cell (B) There are 44 meaningless and 20 codons for amino acids (C) There are 64 amino acids for coding (D) Genetic code is triplet

//X

(D) Genetic code is triplet

//M1//QN124//MCQ//DL0

Which one of the following pairs is correctly matched with regard to the codon and the amino acid coded by it?

(A) UUA-Valine (B) AAA-Lysine (C) AUG-Cysteine (D) CCC-Alanine

//X

(B) AAA-Lysine

//M1//QN125//MCQ//DL0

Which one of the following is not correct option for t − RNA?

(A) It has and anticodon (B) It has an amino acid acceptor end. (C) t − RNAs are not specific for each amino acid. (D) There are no t − RNAs for stop codons.

//X

(C) t − RNAs are not specific for each amino acid.

//M1//QN126//MCQ//DL0

________ is common for both DNA and RNA but ________ is present only in DNA

(A) Uracil, thymine (B) Cytosine, Uracil (C) Guanine, thymine (D) cytosine, uracil

//X

(C) Guanine, thymine

//M1//QN127//MCQ//DL0

Which one of the following pairs is correctly matched with regards to the codon and the amino acid coded by it?

(A) UUA − valine (B) AUG – lysine (C) AUG − cysteine (D) CCC − alanine

//X

(A) UUA − valine

//M1//QN128//MCQ//DL0

Which is stop codon?

(A) CUA (B) AUG (C) UGG (D) UAG

//X

(D) UAG

//M1//QN129//MCQ//DL0

Genetic code consists of

(A) 4 codons, each with two nucleotides (B) 16 codons, each with four nucleotides (C) 64 codons, each with two nucleotides (D) 64 codons, each with three nucleotides

//X

(D) 64 codons, each with three nucleotides

//M1//QN130//MCQ//DL0

Which RNA contains information for synthesis of protein?

(A) m-RNA (B) r-RNA (C) t-RNA (D) All of the above

//X

(A) m-RNA

//M1//QN131//MCQ//DL0

A unit of three successive nucleotides indicates the position of particular ________in the constitution of protein.

(A) Monomer of carbohydrates (B) Fatty acids (C) Nucleotides (D) Amino acids

//X

(D) Amino acids

//M1//QN132//MCQ//DL0

The Sequence of nitrogen bases in a particular region of DNA molecule was found to be CAG, CCC, GAT. What would be the sequence of nitrogen bases in the m-RNA?

(A) CAG, CCC, CAU (B) GUC, GGG, CUA (C) GUC, CCC, GAT (D) GAC, TAG, CTA

//X

(B) GUC, GGG, CUA

//M1//QN133//MCQ//DL0

Main components for translation process are ___.

(A) ribosome, t-RNA, amino acids (B) ribosome m-RNA, DNA (C) DNA m-RNA, t-RNA (D) ribosome, cytoplasm, t-RNA, r-RNA

//X

(A) ribosome, t-RNA, amino acids

//M1//QN134//MCQ//DL0

Which one of the following amino acids always initiate the synthesis of translation?

(A) Serine (B) Valine (C) Methionine (D) Tryptophan

//X

(C) Methionine

//M1//QN135//MCQ//DL0

Which enzyme is essential for transportation of amino acids ?

(A) m-RNA synthetase (B) DNA polymerase-III (C) Transcriptase (D) t-RNA synthetase

//X

(D) t-RNA synthetase

//M1//QN136//MCQ//DL0

The code of nitrogen bases in a particular region of m-RNA is AUG, then the anticodon will be on t-RNA is ________.

(A) AUC (B) TAC (C) UAC (D) TAG

//X

(C) UAC

//M1//QN137//MCQ//DL0

Out of the following which has anticodon?

(A) m-RNA (B) DNA (C) r-RNA (D) t-RNA

//X

(D) t-RNA

//M1//QN138//MCQ//DL0

Which is helpful in the process of elongation?

(A) GTP as energy source, t-RNA synthetase. (B) GTP as energy source, elongation factors. (C) ATP as energy source, elongation factors. (D) ATP as energy source, t-RNA synthetase.

//X

(B) GTP as energy source, elongation factors.

//M1//QN139//MCQ//DL0

The ribosome moves along m-RNA in 3' direction by distance of ________.

(A) Two codons (B) Three codons (C) Four codons (D) One codon

//X

(D) One codon

//M1//QN140//MCQ//DL0

What is the function of non-sense codon?

(A) It translocate m-RNA to cytoplasm from DNA. (B) Release the synthesized polypeptide chain from ribosomes. (C) It carrying specific amino acid molecule on m-RNA. (D) It release polypeptide chain from methionine.

//X

(B) Release the synthesized polypeptide chain from ribosomes.

//M1//QN141//MCQ//DL0

Who can directly code for synthesis of proteins?

(A) DNA (B) RNA (C) Gene (D) Nucleotide

//X

(B) RNA

//M1//QN142//MCQ//DL0

The process of translation of mRNA to proteins begins as soon as :

(A) The larger subunit of ribosome encounters mRNA (B) Both the subunits join together to bind with mRNA (C) The tRNA is activated and the larger subunit of ribosome encounters mRNA (D) The small subunit of ribosome encounters mRNA

//X

(D) The small subunit of ribosome encounters mRNA

//M1//QN143//MCQ//DL0

Polysome is formed by

(A) a ribosome with several subunits (B) ribosomes attached to each other in a linear arrangement (C) several ribosomes attached to a single mRNA (D) many ribosomes attached to a strand of endoplasmic reticulum.

//X

(C) several ribosomes attached to a single mRNA

//M1//QN144//MCQ//DL0

Expressed Sequence Tages (ESTs) refers to

(A) Genes expressed as RNA (B) Polypeptide expression (C) DNA polymorphism (D) None of the above.

//X

(A) Genes expressed as RNA

//M1//QN145//MCQ//DL0

The first phase of translation is

(A) Recognition of an anti-codon (B) Binding of mRNA to ribosome (C) Recognition of DNA molecule (D) Aminoacylation of tRNA

//X

(D) Aminoacylation of tRNA

//M1//QN146//MCQ//DL0

In mRNA, UTRs are present at

(A) 5' end before start codon only (B) Both ends before start codon and after stop codon (C) 3' end after stop codon only (D) 5'end after start codon and 3' end before stop codon

//X

(B) Both ends before start codon and after stop codon

//M1//QN147//MCQ//DL0

Choose the correct statement

(A) Transcription and translation occur in same compartment for prokaryotes (B) Monocistonic RNA - more than one structural genes under single promoter (C) Introns and exons both code for protein synthesis (D) In prokaryotes, splicing and tailing occurs before translation.

//X

(A) Transcription and translation occur in same compartment for prokaryotes

//M1//QN148//MCQ//DL0

Which of the following step of translation does not consume a high energy phosphate bond?

(A) Translocation (B) Amino acid activation (C) Peptidyl transferase reaction (D) Aminoacyl tRNA binding to A-site

//X

(A) Translocation

//M1//QN149//MCQ//DL0

The process in which amino acids are activated in the presence of ATP and linked to their cognate tRNA is commonly called

(A) Aminoacylation of tRNA (B) Aminoacylation of amino acid (C) Discharging of tRNA (D) Charging of mRNA

//X

(A) Aminoacylation of tRNA

//M1//QN150//MCQ//DL0

In mRNA, untranslated regions (UTRs) are required for

(A) Efficient translation process (B) Synthesising rRNA (C) Sequencing the codes for polypeptide formation (D) Binding of ribosome with tRNA

//X

(A) Efficient translation process

//M1//QN151//MCQ//DL0

In an inducible operon, the genes are

(A) usually not expressed unless a signal turns them “on”. (B) usually expressed unless a signal turns them “off”. (C) never expressed (D) always expressed.

//X

(A) usually not expressed unless a signal turns them “on”.

//M1//QN152//MCQ//DL0

Who discovered a lac-operon?

(A) Jacob and Monad (B) Watson and Crick (C) Gamov and Khorana (D) Avery and McCarty

//X

(A) Jacob and Monad

//M1//QN153//MCQ//DL0

Name the gene which can produce a product which will block operator.

(A) Promoter gene (B) Structural gene (C) Repressor gene (D) Control gene

//X

(C) Repressor gene

//M1//QN154//MCQ//DL0

The Repressor protein is a product of ________.

(A) promoter gene (B) repressor gene (C) operator gene (D) structural gene

//X

(B) repressor gene

//M1//QN155//MCQ//DL0

The sugar which was used by Jacob and Monad for explanation of regulation of gene expression is...

(A) glucose (B) lactose (C) sucrose (D) Both (A) and (B)

//X

(D) Both (A) and (B)

//M1//QN156//MCQ//DL0

Which bacteria has been used regarding regulation of gene expression ?

(A) Streptococcus (B) Bacillus (C) E-coli (D) Salmonella

//X

(C) E-coli

//M1//QN157//MCQ//DL0

Which enzyme is necessary to break down the lactose into Glucose and galactose ?

(A) β-glucosidase (B) Permease (C) Transacetylase (D) All of the above

//X

(D) All of the above

//M1//QN158//MCQ//DL0

The segment of DNA which exercises a control of an enzyme which is responsible for digestion of lactose is ________.

(A) Operator gene (B) Promoter gene (C) Structural gene (D) Regulator gene

//X

(A) Operator gene

//M1//QN159//MCQ//DL0

Which is responsible for switching on the lac-operon in bacteria?

(A) lactose (B) numbers of bacteria (C) structural gene (D) RNA polymerase

//X

(A) lactose

//M1//QN160//MCQ//DL0

Operon contains :

(A) Structural gene + promoter gene + operator gene (B) Regulator gene + operator gene (C) Regulator gene + promoter gene + operator gene (D) Regulator gene + promoter gene+ operator gene+ structural gene

//X

(D) Regulator gene + promoter gene+ operator gene+ structural gene

//M1//QN161//MCQ//DL0

It controls the rate of m-RNA synthesis.

(A) Operator gene (B) Regulator gene (C) Promoter gene (D) Structural gene

//X

(C) Promoter gene

//M1//QN162//MCQ//DL0

It exercises a control over transcription.

(A) Operator gene (B) Promoter gene (C) Termination gene (D) Regulator gene

//X

(A) Operator gene

//M1//QN163//MCQ//DL0

In operon concept, regulatory gene functions as

(A) Repressor (B) Regulator (C) Inhibitor (D) All of these

//X

(A) Repressor

//M1//QN164//MCQ//DL0

In lac - operon if mutation occurs in the y gene of the 'Structural gene' then ________.

(A) Permease will not be synthesized. (B) b-Galactosidase will not be synthesized. (C) Transacetylase will not be synthesized (D) Lactose digestion will be rapid.

//X

(A) Permease will not be synthesized.

//M1//QN165//MCQ//DL0

Genes that are involved in turning on or off the transcription of a set of structural genes are called

(A) Polymorphic genes (B) Operator genes (C) Redundant genes (D) Regulatory genes

//X

(B) Operator genes

//M1//QN166//MCQ//DL0

At what level gene expression does not occur?

(A) Formation of primary transcript (B) Processing level (C) Translation level (D) Replication level

//X

(D) Replication level

//M1//QN167//MCQ//DL0

Which one of the following is not a part of a transcription unit in DNA ?

(A) The inducer (B) A terminator (C) A promoter (D) The structural gene

//X

(A) The inducer

//M1//QN168//MCQ//DL0

Match the following genes of the Lac operon with their respective products. Select the correct option.

Column : I | Column : II
a | i gene | i | b−galactosidase
b | z gene | ii | Permease
c | a gene | iii | Repressor
d | y gene | iv | Transacetylase

(A) a – i, b – iii, c – ii, d – iv (B) a – iii, b – i, c – ii, d – iv (C) a – iii, b – i, c – iv, d – ii (D) a – iii, b – iv, c – i, d – ii

//X

(C) a – iii, b – i, c – iv, d – ii

//M1//QN169//MCQ//DL0

Y gene in lac operon responsible for _____.

(A) Codes for b - galactosidase (B) Primarily responsible for hydrolysis of the disaccharide (C) Encodes for transacetylase. (D) Codes for permease.

//X

(D) Codes for permease.

//M1//QN170//MCQ//DL0

In Operon concept, the regulator gene regulates chemical reactions in the cell by

(A) Inactivating enzymes in the reaction (B) Inhibiting transcription of mRNA (C) Inhibiting migration of mRNA into cytoplasm (D) Inhibiting the substrate in the reaction

//X

(A) Inactivating enzymes in the reaction

//M1//QN171//MCQ//DL0

Regulation of lac operon by repressor is referred to as

(A) Positive regulation (B) Induced regulation (C) Negative regulation (D) Autoregulation

//X

(C) Negative regulation

//M1//QN172//MCQ//DL0

Select the two correct statements out of the four (i - iv) statements given below about lac operon (i) Glucose or galactose may bind with the repressor and inactivate it (ii) In the absence of lactose, the repressor binds with the operator region. (iii) The z gene codes for permease. (iv) This was elucidated by Francois Jacob and Jacques Monod. The correct statements are

(A) (ii) and (iii) (B) (i) and (iii) (C) (ii) and (iv) (D) (i) and (ii)

//X

(C) (ii) and (iv)

//M1//QN173//MCQ//DL0

Genes that are involved in turning on or off the transcription of a set of structural genes are called

(A) Polymorphic genes (B) Operator genes (C) Redundant genes (D) Regulatory genes

//X

(B) Operator genes

//M1//QN174//MCQ//DL0

Wild type E.coli cells are growing in normal medium with glucose. There are transferred to a medium containing only lactose as the sugar. Which one of the following changes take place ?

(A) The lac-operon is prepressed (B) All Operons are induced (C) E.coil cells stop dividing (D) The lac-Operon is induced

//X

(D) The lac-Operon is induced

//M1//QN175//MCQ//DL0

In lac operon, lactose works as inducer as

(A) It is responsible for the binding of repressor to the operator region (B) In presence of lactose there is transcription of structural gene (C) It initiates the transcription of repressor mRNA (D) It prevents RNA polymerase from transcribing the operon

//X

(B) In presence of lactose there is transcription of structural gene

//M1//QN176//MCQ//DL0

It is not a HGP goal.

(A) To prepare a genetic map. (B) The deciphering of nucleotide sequence and store is referred to data base. (C) To understand ELSI. (D) To understand genetic mutation

//X

(D) To understand genetic mutation

//M1//QN177//MCQ//DL0

When was HGP completed?

(A) April - 2001 (B) July - 2003 (C) April - 2000 (D) April - 2003

//X

(D) April - 2003

//M1//QN178//MCQ//DL0

Human Genome size is ________.

(A) > 3 billion (B) > 5 billion (C) > 10 billion (D) > 1 billion

//X

(A) > 3 billion

//M1//QN179//MCQ//DL0

The deciphering of nucleotide sequence and its store is referred to as ________.

(A) genome (B) data size (C) data base (D) genetic map

//X

(C) data base

//M1//QN180//MCQ//DL0

How much genome codes for proteins?

(A) 10% (B) 2% (C) 99% (D) 50%

//X

(B) 2%

//M1//QN181//MCQ//DL0

In humans most genes are present on which chromosome.

(A) first chromosome (B) fifth chromosome (C) X chromosome (D) Y chromosome

//X

(A) first chromosome

//M1//QN182//MCQ//DL0

In humans, fewest genes are present on chromosome ________ .

(A) X (B) Y (C) First (D) Both X and Y

//X

(B) Y

//M1//QN183//MCQ//DL0

How many gene are present on Y chromosom e?

(A) 231 (B) 2968 (C) 3000 (D) 900

//X

(A) 231

//M1//QN184//MCQ//DL0

Chromosome 1 has _______ genes Y has the ____.

(A) 2768, 236 (B) 2968, 231 (C) 2163, 336 (D) 1753, 721

//X

(B) 2968, 231

//M1//QN185//MCQ//DL0

The total number of nitrogenous bases in human genome is estimated to be about

(A) 3.5 million (B) 35 thousand (C) 35 million (D) 3.1 billion

//X

(D) 3.1 billion

//M1//QN186//MCQ//DL0

Human Genome Project (HGP) is closely associated with the rapid development of a new area in biology called as

(A) biotechnology (B) bioinformatics (C) biogeography (D) bioscience

//X

(B) bioinformatics

//M1//QN187//MCQ//DL0

Identify the following statements as true (T) or false (F) w.r.t. goals of Human Genome Project and select the correct option. A : Address the ethical, legal and social issues that may arise from the project. B : Identify approximately 3 billion genes in human DNA.

| A | B
(A) | F | F
(B) | T | T
(C) | F | T
(D) | T | F

(A)

F

F

(B)

T

T

(C)

F

T

(D)

T

F

//X

(D)

T

F

//M1//QN188//MCQ//DL0

Select the incorrect match w.r.t Human Genome Project.

(A) SNP – Single nucleotide polymerase (B) BAC – Bacteria artificial chromosome (C) YAC – Yeast artificial chromosome (D) ELSI – Ethical, legal and social issues

//X

(A) SNP – Single nucleotide polymerase

//M1//QN189//MCQ//DL0

Read the following statements and select the correct option. A : Less than 2 percent of human genome codes for proteins. B : Chromosome Y in humans has the fewest genes.

(A) Only statement A is correct (B) Only statement B is correct (C) Both the statements are correct (D) Both the statements are incorrect

//X

(C) Both the statements are correct

//M1//QN190//MCQ//DL0

Identify the following statements as true (T) or false (F) and select the correct option. A : Frederick Sanger is credited for developing the method for determination of amino acid sequences in proteins. B : DNA of Drosophila has not yet been sequenced till now.

| A | B
(A) | T | T
(B) | F | F
(C) | F | T
(D) | T | F

(A)

T

T

(B)

F

F

(C)

F

T

(D)

T

F

//X

(D)

T

F

//M1//QN191//MCQ//DL0

Which of the following is correct about DNA segment?

(A) Code for proteins (B) Intervening sequences (C) VNTR (D) All of the above

//X

(D) All of the above

//M1//QN192//MCQ//DL0

In DNA fingerprinting, separation of DNA fragments occur by ________.

(A) Gas - chromatography (B) TLC (C) Electrophoresis (D) PCR

//X

(C) Electrophoresis

//M1//QN193//MCQ//DL0

It is known as DNA scissors.

(A) RE (Restriction Enzyme) (B) Ligase (C) DNA polymerase (D) RNA polymerase

//X

(A) RE (Restriction Enzyme)

//M1//QN194//MCQ//DL0

Who discovered DNA fingerprinting ?

(A) Alec Jeffery (B) Jacob Monad (C) Herbert Boyer (D) Stanley Cohen

//X

(A) Alec Jeffery

//M1//QN195//MCQ//DL0

What is the use of radioactive DNA probe ?

(A) The DNA probe binds to specific DNA sequence on the nylon membrane. (B) Separation of DNA fragments. (C) It makes x-ray film. (D) Extraction of DNA fragments.

//X

(A) The DNA probe binds to specific DNA sequence on the nylon membrane.

//M1//QN196//MCQ//DL0

Satellite DNA is a useful tool for

(A) Organ transplantation (B) Gender detection (C) Forensic science (D) Mutation detection

//X

(C) Forensic science

//M1//QN197//MCQ//DL0

The grouping of human chromosomes is based on

(A) Secondary constrictions alone (B) Dot-like satellites aone (C) Banding patterns alone (D) All of the above

//X

(C) Banding patterns alone

//M1//QN198//MCQ//DL0

What is it that forms the basis of DNA fingerprinting?

(A) The relative proportions of purines and pyrimidnes in DNA (B) The relative difference in the DNA occurrence in blood, skin and saliva. (C) The relative amount of DNA in the ridges and grooves of the fingerprints. (D) Satellite DNA occurring as highly repeated short DNA segments.

//X

(D) Satellite DNA occurring as highly repeated short DNA segments.

//M1//QN199//MCQ//DL0

When does radioactive DNA pattern become visible?

(A) While bombarding with DNA probes. (B) While radioactive DNA pattern is transferred to X-ray film by direct exposure. (C) While washing DNA fragments. (D) While DNA fragments are separated by electrophoresis.

//X

(B) While radioactive DNA pattern is transferred to X-ray film by direct exposure.

//M1//QN200//MCQ//DL0

DNA polymorphism forms the basis of :

(A) DNA finger printing (B) Both genetic mapping and DNA finger printing (C) Translation (D) Genetic mapping

//X

(B) Both genetic mapping and DNA finger printing

//M1//QN201//MCQ//DL0

Satellite DNA is important because it

(A) does not code for proteins and is same inall members of the population (B) codes for enzymes needed for DNA-replication (C) codes for proteins needed in cell cycle (D) shows high degree of polymorphism in population and also the same degree of polymorphism in an individual, which is heritable from parents to children.

//X

(D) shows high degree of polymorphism in population and also the same degree of polymorphism in an individual, which is heritable from parents to children.

//M1//QN202//MCQ//DL0

The technique of DNA fingerprinting involves use of __A__ as __B__ that shows very high degree of polymorphism. The correct option for A and B is

(A) | A - satellite DNA, | B - probe
(B) | A - repetitive DNA, | B - coding DNA
(C) | A - probe, | B - polymerase
(D) | A - short tandem repeats, | B - repetitive DNA

(A)

A - satellite DNA,

B - probe

(B)

A - repetitive DNA,

B - coding DNA

(C)

A - probe,

B - polymerase

(D)

A - short tandem repeats,

B - repetitive DNA

//X

(A)

A - satellite DNA,

B - probe

//M1//QN203//MCQ//DL0

All of the following are the basis for classifying satellite DNA into different categories, except

(A) Length of segment (B) Presence of repetitive units (C) Base composition (D) Number of repetitive units

//X

(B) Presence of repetitive units

//M1//QN204//MCQ//DL0

Polymorphism in DNA arises due to

(A) Translation (B) DNA sequencing (C) Transcription (D) Mutation

//X

(D) Mutation

//M1//QN205//MCQ//DL0

Assertion : Replication and transcription occur in the nucleus but translation takes place in the cytoplasm. Reason : mRNA is transferred from the nucleus into cytoplasm where ribosomes and amino acids are available for protein synthesis.

(A) A and R both are correct, and R is correct explanation of A. (B) A and R both are correct, but R is not correct explanation of A. (C) A is correct, but R is wrong (D) A is wrong, but R is correct

//X

(A) A and R both are correct, and R is correct explanation of A.

//M1//QN206//MCQ//DL0

Assertion : m-RNA has terminator codons at the end. Reason : Terminator codons are present on m-RNA otherwise protein synthesis will not be completed.

(A) A and R both are correct, and R is correct explanation of A. (B) A and R both are correct, but R is not correct explanation of A. (C) A is correct, but R is wrong (D) A is wrong, but R is correct

//X

(A) A and R both are correct, and R is correct explanation of A.

//M1//QN207//MCQ//DL0

Assertion : AUG is the first codon in the transcription process. Reason : AUG is an initiation codon.

(A) A and R both are correct, and R is correct explanation of A. (B) A and R both are correct, but R is not correct explanation of A. (C) A is correct, but R is wrong (D) A is wrong, but R is correct

//X

(A) A and R both are correct, and R is correct explanation of A.

//M1//QN208//MCQ//DL0

Assertion : In eukaryotes, hnRNA is formed at the end of transcription. Reason : In eukaryotes genes are interrupted.

(A) A and R both are correct, and R is correct explanation of A. (B) A and R both are correct, but R is not correct explanation of A. (C) A is correct, but R is wrong (D) A is wrong, but R is correct

//X

(A) A and R both are correct, and R is correct explanation of A.

//M1//QN209//MCQ//DL0

Assertion : New strand of DNA in replication is synthesized with the help of RNA polymerase. Reason : RNA polymerase forms short strand of RNA which is complementary to DNA-template which joins DNA at initiation site - this is called primer.

(A) A and R both are correct, and R is correct explanation of A. (B) A and R both are correct, but R is not correct explanation of A. (C) A is correct, but R is wrong (D) A is wrong, but R is correct

//X

(D) A is wrong, but R is correct

//M1//QN210//MCQ//DL0

Assertion : Avery and McCarty proved that DNA replication is semiconservative. Reason : In replication of DNA, DNA ligase joins the DNA fragments with phosphodiester bond.

(A) A and R both are correct, and R is correct explanation of A. (B) A and R both are correct, but R is not correct explanation of A. (C) A is correct, but R is wrong (D) A is wrong, but R is correct

//X

(D) A is wrong, but R is correct

//M1//QN211//MCQ//DL0

Assertion : Genetic codon refers to a sequence of nitrogen bases on m-RNA. Reason : A genetic codon encodes information for the protein synthesis.

(A) A and R both are correct, and R is correct explanation of A. (B) A and R both are correct, but R is not correct explanation of A. (C) A is correct, but R is wrong (D) A is wrong, but R is correct

//X

(B) A and R both are correct, but R is not correct explanation of A.

//M1//QN212//MCQ//DL0

Assertion : DNA replication takes place in the S-phase. Reason : DNA replication is semiconservative type.

(A) A and R both are correct, and R is correct explanation of A. (B) A and R both are correct, but R is not correct explanation of A. (C) A is correct, but R is wrong (D) A is wrong, but R is correct

//X

(B) A and R both are correct, but R is not correct explanation of A.

//M1//QN213//MCQ//DL0

Assertion : DNA polymerase breaks hydrogen bond between DNA strands in the process of transcription. Reason : In bacterial cell, one type of DNA polymerase is seen.

(A) A and R both are correct, and R is correct explanation of A. (B) A and R both are correct, but R is not correct explanation of A. (C) A is correct, but R is wrong (D) A is wrong, but R is correct

//X

(D) A is wrong, but R is correct

//M1//QN214//MCQ//DL0

Assertion : Catalytic functions were assigned to RNA molecule during evolution. Reason : The rate of mutation is quite fast in RNA.

(A) A and R both are correct, and R is correct explanation of A. (B) A and R both are correct, but R is not correct explanation of A. (C) A is correct, but R is wrong (D) A is wrong, but R is correct

//X

(B) A and R both are correct, but R is not correct explanation of A.

//M1//QN215//MCQ//DL0//EQ

Identify x and y in a given diagram.

x
y
Y - H1 Histone
Y - Histone octamer

(A) X - H1 Histone, Y = DNA (B) X - DNA, Y - H1 Histone (C) X - Histone octamer, (D) X - DNA,

//X

(B) X - DNA, Y - H1 Histone

//M1//QN216//MCQ//DL0//EQ

Which stage is represented by following diagram of transcription?

3'
5'
σ
5'
3'

(A) Elongation (B) Initiation (C) Termination (D) Both (B) and (C)

//X

(A) Elongation

//M1//QN217//MCQ//DL0//EQ

What is X and Y in a given diagram.

(A) Replication, Protein (B) Cell division, Protein (C) Replication, Amino acid (D) t-RNA, r-RNA

//X

(A) Replication, Protein

//M1//QN218//MCQ//DL0//EQ

The DNA replication diagram is given, choose the correct option regarding this.

(A) Strand (i) Shows direction of DNA replication (B) Strand (ii) Shows direction of DNA replication (C) Strand (i) Shows direction of DNA replication for leading strand. (D) Strand (ii) Shows direction of DNA replication for leading strand.

//X

(C) Strand (i) Shows direction of DNA replication for leading strand.

//M1//QN219//MCQ//DL0//EQ

Which process is represented by given diagram?

(A) Replication (B) Transcription (C) Translation (D) DNA fingerprinting

//X

(C) Translation

//M1//QN220//MCQ//DL0//EQ

Which process is shown by given diagram ?

(A) Replication in prokaryotes (B) Transcription in prokaryotes (C) Replication in eukaryotes (D) Transcription in eukaryotes

//X

(D) Transcription in eukaryotes

//M1//QN221//MCQ//DL0//EQ

Identify 'x' in a given diagram.

(A) Terminator (B) Template strand (C) RNA polymerase (D) Promoter

//X

(D) Promoter

//M1//QN222//MCQ//DL0//EQ

What is in a given diagram ?

(A) Inducer (B) Repressor (C) Regulatory gene (D) m-RNA

//X

(A) Inducer

//M1//QN223//MCQ//DL0//EQ

What is E, F, G and H in a given diagram ?

| E | F | G | H
(A) | β - galacto sidase | Permease | Trans-acetylase | Lactose
(B) | Permease | β - galacto sidase | Trans-acetylase | Lactose
(C) | Lactose | β - galacto sidase | Permease | Transacetylase
(D) | Lactose | Trans-acetylase | Permease | β - galacto sidase

(A)

β -

galacto sidase

Permease

Trans-acetylase

Lactose

(B)

Permease

β -

galacto sidase

Trans-acetylase

Lactose

(C)

Lactose

β -

galacto sidase

Permease

Transacetylase

(D)

Lactose

Trans-acetylase

Permease

β -

galacto sidase

//X

(C)

Lactose

β -

galacto sidase

Permease

Transacetylase

//M1//QN224//MCQ//DL0//EQ

Identify the given model.

5'
GC
GC
A
TA
T
TA
G
GC
AT
CG
AT
C
T
A
GC
AT
TA
CG
GC
AT
AT
GC
T
CG
TA
A
CG
AT
GC
AT
G
C
TA
TA
A
T
GC
GC
3'
conservative DNA
replication
for semiconservative
DNA replication
for semi-conservative
DNA transcription
for conservative
DNA transcription

(A) Watson-Crick model for (B) Watson-Crick model (C) Watson-Crick model (D) Watson-Crick model

//X

(B) Watson-Crick model

//M1//QN225//MCQ//DL0//EQ

Which of the following diagram is correct regarding DNA replication ?

(A) (B) (C) (D)

//X

(D)

//M1//QN226//MCQ//DL0//EQ

The given diagram shows ________

Y
Z

(A) Nucleoid (B) Nucleosome (C) Chromatin material (D) Histone octamer

//X

(B) Nucleosome

//M1//QN227//MCQ//DL0//EQ

Which experiment is shown by given diagram?

(A) Reproduction in bacteria (B) Reproduction of virus (C) Viral DNA / genetic material pass in to bacteria (D) Viral capsid passes in to bacteria

//X

(C) Viral DNA / genetic material pass in to bacteria

//M1//QN228//MCQ//DL0//EQ

What is 'A' in a given diagram ?

A

(A) Phosphate (B) Sugar (C) Phosphate-Sugar (D) Base pair

//X

(C) Phosphate-Sugar

//M1//QN229//MCQ//DL0//EQ

Nucleotide structure is given below. Which bonds are presented by P and Q ?

(A) P-N-Glycosidic bond, Q-phosphoester bond (B) P-Phosphoester bond, Q-N - Glycosidic bond (C) P-Phosphodiester bond, Q-N-Glycosidic bond (D) N-Glycosidic bond, Q-Phosphodiester bond

//X

(A) P-N-Glycosidic bond, Q-phosphoester bond

//M1//QN230//MCQ//DL0//EQ

The Hershey - Chase experiment is given. What processes are shown by P, Q and R ?

//X

(answer not detected)