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Embryo-sac in angiosperm : The nucleus of the functional megaspore divides mitotically to form two nuclei which move to the opposite poles, forming the 2- nucleate embryo sac. Two more sequential mitotic nuclear divisions result in the formation of the 4-nucleate and later the 8-nucleate stages of the embryo sac. It is of interest to note that these mitotic divisions are strictly free nuclear, that is, nuclear divisions are not followed immediately by cell wall formation. After the 8-nucleate stage, cell walls are laid down leading to the organisation of the typical female gametophyte or embryo sac. Observe the distribution of cells inside the embryo sac. Six of the eight nuclei are surrounded by cell walls and organised into cells; the remaining two nuclei, called polar nuclei are situated below the egg apparatus in the large central cell. There is a characteristic distribution of the cells within the embryo sac. Three cells are grouped together at the micropylar end and constitute the egg apparatus. The egg apparatus, in turn, consists of two synergids and one egg cell. The synergids have special cellular thickenings at the micropylar tip called filiform apparatus, which play an important role in guiding the pollen tubes into the synergid. Three cells are at the chalazal end and are called the antipodals. The large central cell, as mentioned earlier, has two polar nuclei. Thus, a typical angiosperm embryo sac, at maturity, though 8-nucleate is 7-celled.
(i) The embryo sac of angiosperm is _____ celled and _____ Nucleate at maturity.(ii) _____out of the eight nuclei are surrounded by cell walls.(iii) Which cells are included in egg apparatus?(iv) How many sequential cell division take place for the formation of 8 nuclei of embryo sac?

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(i) 7 and 8 Nucleate (ii) 6 - Nuclei (iii) Egg apparatus contains one egg and two synergid cells. (iv) 3 mitosis